Figure 6. Ameloblast-lineage cells had increased p-PKC and SATB1 when they were exposed to fluoride.

A) Densitometry analysis of protein extracted from human ameloblast-lineage cells grown in media supplemented with NaF or AlF₃ shows significant increase in SABT1 protein when cells were exposed to either 10 or 100 µM fluoride as compared to control. One way ANOVA shows P<0.05, Tukey’s multiple comparison post-test shows * is significantly different from the control samples (n = 4). B) The Western blot analysis showed that OAG, the analog of DAG, at concentration of 62.5 µM or 125 µM significantly increased the phosphorylated PKC, which served as a positive control. NaF at 10 µM or 100 µM also increased the levels of phosphorylated PKC in ameloblast-lineage cells, though at the moderate levels as compared to OAG treated cells. Either OAG or NaF did affect the levels of PKC.