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. 2014 Aug 4;9(8):e104000. doi: 10.1371/journal.pone.0104000

Figure 4. Alternative splicing of TRPM3 transcripts in the human lens.

Figure 4

(A) RT-PCR showing presence of transcripts for variant 9 (exon 1–5, 411 bp), variants 1–8 (exon 2–5, 414 bp) and a novel lens variant, KF987075 (EST - exon 5, 403 bp). (B) Quantitative RT-PCR showing relative copy numbers of TRPM3 variant 9 (2.06E+01, SD 9.46E+00), variants 1–8 combined (2.76E+05, SD 2.15E+04), and lens variant KF987075 (3.17E+04, SD 8.07E+03) standardized against RPL19. TRPM3 primers used for amplification were as follows; RT1F - RT5R for variant 9, RT2F - RT5R for variants 1–8, and ESTF - RT5R for lens variant KF987075 (Table S5).