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. 2014 Aug 4;9(8):e104039. doi: 10.1371/journal.pone.0104039

Figure 6. Dependence of foam cell formation in MDMs on TLR8 activation by HIV ssRNA.

Figure 6

(A) Intracellular expression of TLR8 in MDMs. MDMs which were incubated with PE-conjugated anti-TLR8 or isotype antibody control. Intracellular expression was determined by flow cytometry. Representative profiles were similar in four independent experiments (n = 4 subjects). (B) Functional silencing of human TLR8 leads to diminution of foam cell formation in MDMs. BODIPY staining of MDMs after pretreatment with TLR8 siRNA or nonsilencing control. Cells were challenged with HIV ssRNA or ssRNA control for 24 h. Bar graph shows integrated fluorescence intensities of BODIPY per cell of the confocal micrographs. Results are representative of four independent experiments with similar results. **p<0.01 compared to NS siRNA + ssRNA41 control.