Figure 1. Histone purification strategies.

Schematic depiction of the workflow of (A) the conventional histone purification method according to Luger and coworkers [2], [3] and (B) our RHP protocol. For further details see the main text. Footnotes indicate variations and simplifications of the initial protocol. * The gel filtration step was successfully omitted in simplified purification schemes [5], [7]–[9]. # These steps can be replaced by dilution into or dialysis against SAU 200 buffer [5]–[9]. ‡ To remove possible DNA contaminations, it was suggested to filter the sample through an anion exchange resin prior to applying it to the cation exchange chromatography [7]–[9]. § Anion exchange filtering and cation exchange chromatography can be combined. See note in step 3.2 and Figure S2 in File S1.