Table 1. Extinction coefficients of Drosophila histones at 280 nm.
Molecular weight (Da)* | ε280 (cm−1 M−1)# | |
H2A | 13,232 | 4,470 |
H2B | 13,565 | 7,450 |
H3 | 15,257 | 4,470 |
H4 | 11,250 | 5,960 |
Octamer | 106,608 | 44,700 |
*Molecular weights do not include the initial methionine.
The extinction coefficients were calculated using the ProtParam tool with water as solvent (Swiss Institute of Bioinformatics; http://web.expasy.org/protparam/) [17].