FIG. 2.

In vitro hydrolysis of nPHB granules. (A) nPHB granules from R. eutropha H16 were incubated for 10 min at 40°C (activation) before the hydrolysis reaction was started by addition of soluble PHB depolymerase. In some experiments the hydrolysis reaction for trypsin-activated nPHB was as fast as the ApdA-activated reaction. (B) nPHB granules from R. eutropha H16 with different additions (no preincubation) were hydrolyzed by addition of soluble PHB depolymerase at 40°C. Note the lag time compared to the data shown in panel A. Reactions with depolymerase or activating compounds alone served as controls. Note the different scales in panels A and B. OD 650nm, optical density at 650 nm.