TABLE 1.
Bacterial strains and plasmidsa
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Escherichia coli DH5α | Cloning strain | |
| E. coli S17-1 | Mobilizing strain | 33 |
| E. coli HMS174 | Host for phaP and/or phaCBA | 17 |
| Ralstonia eutropha H16 | Source of PHB | DSMZ428 |
| Bacillus megaterium | Source of PHB | DSMZ32 |
| Bacillus cereus | Source of PHB | DSMZ31 |
| Chromobacterium violaceum | Source of PHASCL | DSMZ30191 |
| Rhodospirillum rubrum S1 | Wild type | DSMZ467 |
| R. rubrum Sm | Spontaneous streptomycin-resistant mutant of S1 (≥600 μg/ml), Smr | This study |
| R. rubrum SmRif | Spontaneous rifampin-resistant mutant of Sm (≥100 μg/ml), Smr Rifr | This study |
| Magnetospirillum gryphiswaldense MSR-1 | Wild type | DSMZ6361 |
| pJoe4036 | Rhamnose-inducible vector for His tagging | 35 |
| pSN2224 | apd in pJoe4036 | This study |
| pEYFP-N1 | eyfp | BD Clontech |
| pEGFP | egfp | BD Clontech |
| pEYFP | eyfp | This study |
| pBBR1-MCS2 | Broad-host-range vector | 19 |
| pJM9238 | phaCBA | 17 |
| pBluescriptKS::phaP | phaP | 17 |
| pSN2338 | Activator-eyfp fusion with lac promotor | This study |
| pSN2343 | Activator-eyfp fusion | This study |
| pSN2372 | Activator-eyfp fusion in pBRR-MCS2 | This study |
| pSN2389 | Activator-eyfp fusion in pBRR-MCS2 with lac promotor | This study |
| pCS11 | mms16-egfp fusion in pBBR1-MCS2 | This study |
a
DSMZ, Deutsche Sammlung für Mikroorganismen and Zellkulturen; PHASCL, short-chain-length PHA.