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. 2014 Aug 4;9(8):e104040. doi: 10.1371/journal.pone.0104040

Figure 4. Myocardial injection of Ad-Ankrd1 in mice promotes cardiac hypertrophy.

Figure 4

Cardiac overexpression of Ankrd1 was achieved by myocardial injection of Ad-Ankrd1 at 2 weeks after TAC, and the mice in each group were sacrificed at 4 weeks after the initial surgery. (A) Representative pictures of Western blot for CARP (upper panel), immunohistochemistry of myocardial CARP (2nd line, scale bar = 50 µm), whole hearts (3rd line, scale bar = 3 mm) and myocyte cross-sectional area stained with rhodamine-conjugated wheat germ agglutinin (low panel, scale bar = 30 µm) from each group. (B) Expression levels of CARP in each group. (C) HW/BW ratio at 4 weeks after surgery in different groups. (D) Myocyte surface area was calculated from 100 cells in each group. (E) ANP gene expression detected by Real-time PCR using β-actin as an internal control. (F) Western blot analysis of calcineurin expression in heart from each group. (G) Semi-quantitative analysis of calcineurin expression. For panel B-E, G, *P<0.01 vs. sham, #P<0.01 vs. TAC + Ad-EGFP, n = 4–9 in each group. Data are mean ± SEM.