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. 2014 Aug 4;9(8):e104040. doi: 10.1371/journal.pone.0104040

Table 2. Sequences of primers for real-time PCR.

Transcripts Forward primer (5′–3′) Reverse primer (5′–3′) Product size (bp)
β-MHC (rat) GCTACCCAACCCTAAGGATGC TCTGCCTAAGGTGCTGTTTCA 196
ANP (rat) CTCCGATAGATCTGCCCTCTTGAA GGTACCGGAAGCTGTTGCAGCCTA 216
β-actin (rat) ATCGTGCGGGACATCAAGG CAGGAAGGAGGGCTGGAACA 180
ANP (mouse) CGGTGTCCAACACAGATC TCTTCTACCGGCATCTTTC 71
β-actin (mouse) TGGACAGTGAGGCAAGGATAG TACTGCCCTGGCTCCTAGCA 121
Calpain 1 (rat) TGCCATGTTCCGTGCTTTCA GACCGTAAAAACGTGGCTGC 178
GAPDH (rat) GATGCCCCCATGTTTGTGAT GGTCATGAGCCCTTCCACAAT 216