Table 2. Sequences of primers for real-time PCR.
Transcripts | Forward primer (5′–3′) | Reverse primer (5′–3′) | Product size (bp) |
β-MHC (rat) | GCTACCCAACCCTAAGGATGC | TCTGCCTAAGGTGCTGTTTCA | 196 |
ANP (rat) | CTCCGATAGATCTGCCCTCTTGAA | GGTACCGGAAGCTGTTGCAGCCTA | 216 |
β-actin (rat) | ATCGTGCGGGACATCAAGG | CAGGAAGGAGGGCTGGAACA | 180 |
ANP (mouse) | CGGTGTCCAACACAGATC | TCTTCTACCGGCATCTTTC | 71 |
β-actin (mouse) | TGGACAGTGAGGCAAGGATAG | TACTGCCCTGGCTCCTAGCA | 121 |
Calpain 1 (rat) | TGCCATGTTCCGTGCTTTCA | GACCGTAAAAACGTGGCTGC | 178 |
GAPDH (rat) | GATGCCCCCATGTTTGTGAT | GGTCATGAGCCCTTCCACAAT | 216 |