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. 2014 Jul 16;13:173. doi: 10.1186/1476-4598-13-173

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Copyright © 2014 Nair et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Stable transfection confirming the ID1 overexpression in A549 cell and dose dependent induction of STMN3 & GSPT1 by ID1 and depletion of ID1 elevates the expression of STMN3 & GSPT1. (A) The overexpressing (OE) clones showed upregulation of STMN3 and GSPT1 in ID1 over expressing clones as compared to the non-targeting control vector (pcDNA3) as observed by RT-PCR, (B) ID1 protein levels were also elevated as seen in a western blot in the selected clones using G418 resistance confirming the ID1 over-expression, (C) ID1 could induce STMN3-luciferase and GSPT1-luciferase promoters in a dose dependent manner in transient transfection experiments in A549 cells, (D) and H1650 cells as shown in the graph.