FIG. 1.

SDS-PAGE analysis of proteins isolated by DNA affinity chromatography. The left lane contains standard proteins, and the right lane contains proteins isolated by their affinity for an oligonucleotide representing the sequence from the center of the ica promoter (MID-ICA). The gel was stained with Coomassie blue. The band intensities in this figure were digitally enhanced by using Adobe PhotoShop for the purpose of clarity. The indicated bands were excised and sequenced by MALDI-TOF mass spectral analysis. The proteins predicted by the sequence obtained are indicated.