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RT-PCR analysis of expression of flaA1 and wbpB in strain NCTC 11637. HP1045, encoding acetyl coenzyme A synthetase, was used as a positive control for constitutive expression. RT, reverse transcriptase. Signals obtained only in the samples where reverse transcriptase was added indicate expression of the genes of interest. Similar results were obtained with strains 26695 and SS1 (data not shown).
