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Gel electrophoresis analysis of the LPS of the flaA1 and wbpB mutants of H. pylori strain NCTC 11637. The LPS were analyzed on SDS-PAGE (12% polyacrylamide) gels (A and B) or 10 to 20% Tricine gradient gels (C) and detected by silver staining (A and C) or Western immunoblotting (B) with anti-Lewis Y antibody (Calbiochem). Only 1/10 of the amount of sample used for the SDS-PAGE gels was loaded on the Tricine gel to allow for good resolution of core bands. WT, wild type.
