FIG. 5.
(A and B) SDS-PAGE analysis of the LPS of knockout mutants and complemented strains detected by Western immunoblotting using anti-Lewis Y (A) or anti-lipid A (B) antibodies. Detection was performed using goat anti-mouse immunoglobulin G conjugated with Alexa Fluor 680 and scanning with a Li-Cor Odyssey infrared imaging system. The anti-Lewis Y antibody detected the O antigen exclusively whereas the anti-lipid A antibody detected the lipid A associated both with the O antigen and with the core LPS. (C) Quantitative data were obtained by normalizing the amount of O antigen detected with either antibody (open bars, anti-Lipid A; hatched bars, anti-LeY) by the amount of core LPS detected with the anti-Lipid A antibody. WT, wild type.