FIG. 6.

SDS sensitivity of the wild-type, mutant, and complemented strains. To compare wild-type and mutant strains, the assay was performed in the presence of kanamycin. Resistance to kanamycin was provided by the pHel3 vector in the wild-type strain. Similar results were obtained when the experiment was performed in the absence of kanamycin, using the plasmid-free wild-type strain (data not shown). To compare the mutants and the complemented strains, the assays were performed in the presence of kanamycin and chloramphenicol. In this case, mutants harboring the pHel2 vector were used as references. (A) ▴, wild type plus pHel3; ▾, flaA1 mutant; ⧫, wbpB mutant. (B) •, flaA1 mutant plus pHel2; ▴, flaA1 mutant plus pHel2-flaA1; ▪, flaA1 mutant plus pHel2-wbpB. (C) ○, wbpB mutant plus pHel2; □, wbpB mutant plus pHel2-wbpB; ▵, wbpB mutant plus pHel2-flaA1.