TABLE 2.
Effect of the inactivation of flaA1 and wbpB on the motility of H. pylori NCTC 11637 as measured by the soft agar plate motility assaya
| OD600 | Diamb (mm) of motility halo of strain:
|
||
|---|---|---|---|
| WT | fla1::kan | wbpB::kan | |
| 0.3 | 9 ± 1 | 2 ± 1 | 3 ± 0 |
| 0.9 | 10 ± 2 | 3 ± 1 | 4 ± 1 |
a
The motility plates were inoculated with two different dilutions of bacterial culture and were typically read after 4 days of growth at 37°C. Longer incubation times did not result in larger motility halos. All cultures exhibited similar viability when plated on BHI-YE. WT, wild type.
b
Results are expressed as means ± standard deviations.