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. 2014 Jun 25;3:e29256. doi: 10.4161/onci.29256

Table 3. Inflammatory cell populations and cytokine expression in inflammatory MSS, non-inflammatory MSS, and MSI colorectal cancers.

  Group 1 (n = 29)   Group 2 (n = 33)   Group 1 vs 2   Group 3 (n = 48)   Group 3 vs 1
Marker Inflammatory MSS CRC(1)   Non-Inflammatory MSS CRC     MSI CRC  
Mean SEM Median Range   Mean SEM Median Range   Ratio p   Mean SEM Median Range   Ratio p
CD3 ct 882 612 701 [65–2491]   685 798 451 [53–3673]   1.29 0.044   1335 1320 905 [13–5604]   1.51 0.391
CD45RO ct 977 787 705 [64–2788]   625 667 521 [0–3362]   1.56 0.055   1462 1031 1359 [78–4595]   1.49 0.031
CD68 im 779 737 618 [50–3678]   601 568 469 [20–2669]   1.29 0.241   908 579 816 [46–2022]   1.17 0.188
CD68 ct 364 276 328 [14–1198]   317 296 249 [20–1274]   1.15 0.378   626 364 596 [43–1464]   1.72 0.002
FoxP3 ct 254 155 223 [5–534]   352 285 255 [54–1162]   0.72 0.378   250 183 206 [15–820]   0.98 0.701
CD8 ct 303 420 155 [5–1956]   225 272 148 [7–1057]   1.35 0.459   717 724 505 [31–2916]   2.36 0.002
CD45RO im 2045 1525 1715 [139–5965]   2326 2651 1574 [99–11293]   0.88 0.553   2716 1620 2614 [125–6462]   1.33 0.083
T-bet ct 105 121 60 [0–533]   124 159 53 [9–664]   0.85 0.554   453 492 233 [4–2053]   4.30 < 0.0001
FoxP3 im 326 321 214 [53–1508]   383 531 177 [0–2568]   0.85 0.603   343 303 259 [0–1159]   1.05 0.761
CD8 im 492 460 345 [40–1806]   579 751 268 [18–3445]   0.85 0.707   837 629 672 [57–3154]   1.70 0.006
CD3 im 1041 936 761 [152–3805]   1308 1621 863 [44–8474]   0.79 0.789   1573 1017 1363 [80–4059]   1.51 0.013
T-bet im 61 61 38 [5–226]   67 84 36 [0–388]   0.91 0.905   115 93 109 [3–386]   1.89 0.005
                                         
IL-16 7.14 3.84 6.48 [1.75–14.05]   11.63 10.02 10.54 [2.08–53.59]   0.61 0.059   14.90 14.89 12.10 [0–87.05]   2.09 0.002
IL-1ra 56.38 90.45 27.83 [7–464.13]   102.11 132.94 44.21 [.27–506.35]   0.55 0.080   105.98 123.77 58.83 [5.6–567.4]   1.88 0.007
CCL5 0.29 0.57 0.12 [0–2.37]   0.49 0.96 0.15 [0–4.29]   0.59 0.438   5.16 18.13 1.37 [0–125.92]   17.79 < 0.0001
CXCL8 12.27 24.75 2.79 [0–114.29]   16.69 50.25 1.51 [0–284.63]   0.74 0.494   77.05 149.22 12.70 [.01–507.6]   6.28 < 0.0001
CXCL9 6.92 8.76 2.66 [0.16–29.43]   5.52 8.86 3.73 [0–49.90]   1.25 0.568   28.51 35.39 14.16 [0–154.09]   4.12 < 0.0001
IL-1β 3.43 12.71 0.09 [0–68.39]   1.2 2.48 0.15 [0–10.58]   2.86 0.577   5.13 8.86 1.78 [0–41.93]   1.50 < 0.0001
CXCL10 6.44 9.98 1.85 [0–44.67]   5.31 8.31 1.54 [0–34.12]   1.21 0.843   61.36 238.43 8.50 [0–1652.31]   9.53 0.003
MIF 107.45 57.42 102.78 [50.73–319.81]   105.96 50.97 87.37 [47.68–226.69]   1.01 0.927   89.23 60.19 76.16 [0–350.63]   0.83 0.084
CXCL1 1.77 2.51 0.89 [0–12.89]   1.99 2.67 1.00 [0–12.80]   0.89 0.978   2.84 3.58 1.40 [0–18.59]   1.60 0.057
1)

Based on the presence of a strong lymphocytic infiltration and/or Crohn’s-like lymphocytic reaction. The intratumoral (ct) and peritumoral (im) densities of CD3, CD8, T-Bet, CD45RO, FoxP3, and CD68 immunoreactive cells were assessed by image analysis of tissue microarray spots and expressed as number of cells/mm2. The amount of each factor was quantified using two multiplex assays and expressed as pg/mL for 25 µg of total protein extracted from MSS or MSI tumor samples containing at least 50% tumor cells. Data were compared with a non-parametric Mann-Whitney test. Abbreviations: CRC, colorectal cancer; MSI, microsatellite instable; MSS, microsatellite stable.