Figure 9.

Western blotting was performed to examine the protein expression of phosphorylated AKT and total-AKT in HUCCT1 cells following transfection with c-ros-oncogene1 (ROS1)-6290 shRNA, or fused-in-glioblastoma (FIG)-363 shRNA, or co-transfection with ROS1-6290 and FIG-363 shRNA. GAPDH was used as an internal reference. Control, cells without any transfection. Negative control (NC), cells transfected with blank vector. ROS+FIG, cells co-transfected with ROS1-6290 and FIG-363 shRNA. ^**P<0.01.