Figure 4. Downregulation of BRD4 impairs hematoendothelial specification. (A and B) Representative flow cytometry dot plots of hematopoietic markers during ESC differentiation on OP9 co-culture. The percentage of CD34⁺/CD31⁺ and CD45⁺ cells corresponding to each dot plot are shown in the upper right corner. (C) Percentage of CD34⁺, CD31⁺, and CD45⁺ cells during hematopoietic differentiation. Primitive hematoendothelial progenitors were identified as CD34⁺/CD31⁺/CD45^-, immature blood cells as CD34⁺/CD45⁺ and mature blood cells as CD45⁺/CD34^-. Percentages are represented as the mean value ± SD of three independent experiments. (D) Colony forming unit (CFU) potential of control, shBRD4, and super-BRD4 ESCs. Results are represented as the mean value ± SD of three independent experiments (*P ≤ 0.05) (**P ≤ 0.01).