Figure 2. Methylation status of human Plgf promoter in normoxic and hypoxic conditions. (A) Schematic representation of the CpG island located in human Plgf promoter at position -388/+156 with respect to the transcription start site. (B) Sequence analysis of bisulfite-treated genomic DNA from HUVECs exposed (bottom) or not (top) to hypoxia for 9 h. Twenty clones for each group have been analyzed. Each row indicates a single clone, whereas each column represents a single CpG site. White circles indicate unmethylated cytosines, whereas black circles represent the methylated ones.