Table 2. Regions of Plgf and Vegf-a genes analyzed by ChIP.
Target | Amplicon # | Region | HRE |
hPlgf | 1 | -9730 / -9570 | |
2 | -4903 / -4747 | ||
3 | -3561 / -3419 | ||
4 | -1702/ -1554 | putative (-1654) | |
5 | -1168 / -1022 | putative (-1047) | |
6 | -350 / -176 | ||
7 | +2208 / +2282 | putative (+2324, +2407, +2422) | |
8 | +8199 / +8305 | ||
9 | +13445 / +13578 | ||
10 | +15976 / +16108 | ||
hVegf | V-PC | -1005 / -868 | active (-978) |
V-NC | -1762 / -1364 | absent | |
V-2-Int | +5161 / +5391 | ||
mPlgf | 11 | -1945 / -1812 | putative (-3100) |
12 | -1001 / -889 | ||
13 | -314 / -202 | ||
14 | +1740 / +1841 | putative (+1767, +2168) | |
15 | +3892 / +4011 | putative (+4030) | |
16 | +6584 / +6708 | putative (+6593) | |
mVegf | V-PC | -944 / -831 | active (-899) |
V-NC | -1903 / -2040 | absent |
The numbers in the region column indicated the area of genes analyzed with respect to transcription start site. In bold are indicated regions in which known and discovered active HRE are located. Numbers in HRE column refer to center position of HREs respect to transcription start site. V-PC, VEGF-A positive control; V-NC, VEGF-A negative control, V-2-Int, region of the second intron of Vegf-a gene where a putative HRE is located.