Table 5. Immunohistochemical analysis and BRAF V600E mutation in individuals with different percentage of hMLH1 methylation.

Gender	Age	hMLH1 methylation %	MLH1 IHCa	N° of methylated genesb	BRAF mutationc
F	80	30.0	-	4	V600E
F	71	0	+	0
F	79	0	+	0
M	87	0	+	1
M	81	83.5	-	4	V600E
F	64	0	+	1
F	82	0	+	2
F	82	78.4	-	5	V600E
F	62	0	+	0
M	81	0	+	2
F	84	51.1	-	2	V600E
F	87	6.0	-	3	V600E
F	85	6.4	+	2
F	70	47.3	-	4	V600E
F	84	0	+	1
M	91	0	-	1
M	87	29.3	-	4
F	79	32.9	-	2
M	80	0	-	1
F	83	76.5	-	4	V600E
F	65	7.3	-	1
M	88	100	-	4	V600E
F	87	75	-	5	V600E
F	77	0	+	2
F	80	14.8	+	3
M	81	0	+	0
F	79	0	+	1
M	80	16.4	+	3	V600E
F	83	0	+	0
M	76	0	+	0

^a IHC = immunohistochemical analysis (+ = positive sample, - = negative sample), ^b Number of methylated genes among the five investigated in the present study, ^c Carriers of the V600E mutation are indicated, wild type subjects are left blank.