Table 6. Primers and annealing temperature (Ta) used during MS-HRM analysis, as well as amplicon length and number of CpG sites for each of the studied genes.
| Gene | Primer sequences: 5′-3′ | Ta | CpG sites | Amplicon lenght |
|---|---|---|---|---|
| APC | F: CGGGGTTTTGTGTTTTATTG R: TCCAACGAATTACACAACTAC |
56 °C | 4 | 71 bp |
| MGMT | F: GCGTTTCGGATATGTTGGGATAAGT R: AACGACCCAAACACTCACCAAA |
58 °C | 12 | 110 bp |
| hMLH1 | F: GGTTATAAGAGTAGGGTTAA R: ATACCAATCAAATTTCTC |
56 °C | 5 | 81 bp |
| RASSF1A | F: TCGGGTTTTATAGTTTTTGTATTTAGGTTTT R: CCTCCCCCAAAATCCAAACTAA |
60 °C | 7 | 87 bp |
| CDKN2A | F: CGGAGGAAGAAAGAGGAGGGGT R: CGCTACCTACTCTCCCCCTCT |
62 °C | 7 | 93 bp |