Figure 4. Attenuation of MST1 expression by MYC through EZH2 and miR-26a/b. (A and B) Analysis of MST1, EZH2 and MYC mRNA levels by RT-qPCR in C4–2 cells after treatment with increasing doses (0, 20, 40, 60 µM) of 10058-F4 for 72h and DMSO (vehicle) or JQ1 (500 nM) for 48h in serum-fed conditions, respectively. (C) Semi-qPCR analysis of miR-26a and miR-26b mRNA levels in C4–2 cells treated either with DMSO (control), 60 µM 10058-F4 or 500 nM JQ1 for 48h in serum-fed conditions. U6 was used as an internal control. (D) Analysis of MST1 and EZH2 mRNA levels by RT-qPCR in C4–2 cells transfected with miR-26a and miR-26b mimics for 48h in serum-fed conditions. Treated cells arbitrary values were normalized to that of the untreated DMSO or Mock control. (E) Analysis of MST1 mRNA levels by RT-qPCR in p69 cells transiently transfected with mock (vector) or MYC expression construct and then treated with DMSO (vehicle) or 5 µM DZNep. (F and G) QPCR analysis of MYC mRNA levels in C4–2 cells treated with DMSO or 5 µM DZNep (G) and with DMSO or 2 µM GSK126 for 72h. Data are (± S.E.) from multiple experiments.