Figure 5.

GPC3 overexpression inhibits tumor growth. The Kaplan-Meier survival curve for each inoculation group represents the percentage of surviving animals at the indicated time points (1×10⁶ cells of each tumor cell line were used to inoculate the peritoneal cavity. n=5/each group) (A). On day 8 after inoculation with tumor cells, mice (10/each group) were sacrificed, peritoneal cavities were opened, and ascitic fluids were collected and analyzed (B). Cancer cells were seeded in a 24-well plate in triplicate and cultured at 37°C. Cell growth was determined at 4, 24, 48, and 72 h using the WST-8 method. Significance was assessed by one-way ANOVA (C). ^**P<0.01, ^*P<0.05. Error bars for B and C, represent SD.