Figure 3.

CFZ alone or combined with CPT-11 blocks the phosphorylation of MEK/ERK and PI3K/AKT pathway, as well as expression of MKP-1 and survivin in SW620 cells. (A) SW620 cells were treated with increasing doses of CFZ (20, 50 and 100 nM) for 48 h. (B) SW620 cells were treated with 100 μM CPT-11 combined with 50 nM CFZ for 48 h. Protein expression was monitored with the indicated antibodies by western blot analysis as described in Materials and methods. Tubulin was used as the loading control.