Figure 7.

Combination effects of CFZ and CPT-11 in a SW620 xenograft model. (A) BALB/c nude mice were subcutaneously inoculated in the back with 10×10⁶ SW620 cells. Once the tumor diameter reached 7 mm, mice were treated with 2.0 mg/kg carfilzomib (i.v., twice weekly for 3 weeks) ± 33 mg/kg CPT-11 (i.p., once weekly for 4 weeks). Tumor volumes were measured twice weekly, and mean tumor volumes were plotted against days of treatment. Bars, SD. ^*P<0.05. (B) H&E staining of tumor tissues from SW620 xenografts after indicated treatment. Magnification, ×200. (C) Representative photomicrographs demonstrating TUNEL staining of SW620 tumor sections after treatment with 2.0 mg/kg CFZ, 33 mg/kg CPT-11, and combination CFZ and CPT-11. Tumors were removed from xenografts and processed according the protocol of the In situ Cell Death Detection Kit, a fluorescent microscope was used to detect TUNEL positive (fluorescent red) cells. (D) After various indicated treatments, the tumors were excised and nuclear protein was extracted. EMSA was carried out for NF-κB activity assay. Oct-1 was used as a loading control.