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. 2014 Jun 25;45(3):1193–1199. doi: 10.3892/ijo.2014.2523

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Copyright © 2014, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Treatment of SUM149PT cancer cells and CD44⁺/CD24^−/Low CSCs with chemotherapeutic agents. (A) Immunofluorescence analysis showing activation of apoptosis in SUM149PT cancer cells and CD44⁺/CD24^−/Low CSCs treated with 1 μM methotrexate or 1 μM SU9516. Cleaved PARP indicating activation of apoptosis was labeled in green. (B) Graph showing the percentage of apoptotic SUM149PT cancer cells and CD44⁺/CD24^−/Low CSCs following treatment with 1 μM methotrexate or 1 μM SU9516. Experiments were performed in triplicate.