TABLE 2.
Primers used to amplify gel shift probesa
| Promoter | Target | PCR primers |
|---|---|---|
| agr P2 | 100 bp upstream of RNAII translational start site | 5′-TAAAATATTAAATACAAATTACATTT-3′ |
| 5′-ATTTTACACCACTCTCCTCA-3′ | ||
| agr P3 | 100 bp upstream of RNAIII translational start site | 5′-TCAACTATTTTCCATCACATC-3′ |
| 5′-ACATAAAAAAATTTACAGTTAAGA-3′ | ||
| spa (protein A) | 100 bp upstream of translational start site | 5′-ATTAATACCCCCTGTATGTA-3′ |
| 5′-AACTTACATTTAAATTTAATTATAA-3′ | ||
| srr (SrrAB) | 100 bp upstream of translational start site | 5′-ACAGGTCATACCTCCCAC-3′ |
| 5′-AGAATTTTTTCACAAAATTTTAG-3′ | ||
| tst (TSST-1) | 100 bp upstream of translational start site | 5′-ATGGTTAATTGATTCATTTAAA-3′ |
| 5′-ATTAGTAATTTTTTATTCATTTTT-3′ | ||
| rpsC (30S ribosomal protein S3) | 100 bp of coding sequence | 5′-TTCATACTGGTAAACCTGG-3′ |
| 5′-TTTGTACACGACGGAAT-3′ |
a
Each experimental probe was designed to incorporate approximately 100 bp upstream of the translational start site for each gene. The control probe consisted of 100 bp of the coding sequence of the rpsC gene. Probes were PCR amplified by using the primers.