Table I.
Primer sequences and detection results of differentially expressed genes identified by qPCR.
| Gene name | Primer direction | Primer sequence (5′-3′) | Product (bp) | Upregulation fold |
|---|---|---|---|---|
| C3 | F-1 | AAAGATAAGAACCGCTGGGAGG | 117 | 20.88 |
| R-1 | CACGACGGGAGGCACAAA | |||
| C5 | F-1 | CCCACTACAGAGGCTACG | 305 | 3.18 |
| R-1 | AGGACTGAGAAACCCAAC | |||
| C4b | F-1 | CTGTCTGCCTACTGGATTGC | 205 | 12.15 |
| R-1 | CCTTCAGGGTTCCTTTGC | |||
| FGA | F-1 | AGGCAACACTTACCACTG | 176 | 26.15 |
| R-1 | GTAATCTCATTTCCACCAG | |||
| FGG | F-1 | ACATTGCCAATAAGGGAG | 209 | 10.15 |
| R-1 | TGTTGTGCCAGTAGGAGA | |||
| FGB | F-1 | TTGCCCATAGAAACGAGG | 153 | 14.82 |
| R-1 | GTGGCGACTTGGAGTGAA | |||
| SELENBP1 | F-1 | CAAAGTATGGCTACAGGG | 84 | 10.16 |
| R-1 | AGTGGCTCTAAGACGATT | |||
| TXNIP | F-1 | CCACCGTCATTTCTAACT | 147 | 20.90 |
| R-1 | ACACCTCCACTATCACCC | |||
| PDZK1IP1 | F-1 | TTTCAGGCGGACACCAAT | 217 | 8.41 |
| R-1 | ACGAGGACCAGGAACACG | |||
| DUSP6 | F-1 | AGCGACTGGAACGAGAAT | 297 | 7.01 |
| R-1 | GTTGGACAGCGGACTACC |
F, forward; R, reverse; C3, complement C3 precursor; C5, complement C5 precursor; C4b, complement 4B preproprotein; FGA, fibrinogen α chain precursor; FGG, fibrinogen γ chain precursor; FGB, fibrinogen β chain precursor; SELENBP1, selenium binding protein 1; TXNIP, thioredoxin-interacting protein; PDZK1IP1, PDZK1-interacting protein 1; DUSP6, dual specificity protein phosphatase 6.