Figure 2.

BMP2 inhibits the cell wound healing capability of human colon cancer cells. Subconfluent HCT116 cells were infected with AdGFP or AdBMP2 for 16 h and reseeded in 6-well plates at ~90% confluency. Upon cell attachment, scratches were made with pipette micro-tips. Floating cells were removed, and the cells were maintained in DMEM containing 1% FBS. The width of the scratched cell gaps were monitored and recorded at the indicated time points. The scratch assay was carried out in triplicate and at least three scratch sites were monitored and recorded in each well. (A) Representative images are shown. (B) Percentages of the wound area closure were measured using ImageJ software. Percentages of average wound closure for each group at different time points were graphed. ^**P<0.001 (BMP2 vs. GFP).