Fig. 3.

Protein carbonylation, poly(ADP-ribose) (PAR) and nicotinamide adenine dinucleotide (NAD) content in the motor cortex of heterozygous (HET) and Ndufs4-null mice. (A) Oxyblot analysis of protein carbonylation in the motor cortex of heterozygous (HET) and knockout (KO) mice at postnatal days 30 (P30) and 50 (P50). (B) Densitometric analysis of oxyblots. Western blotting evaluation of PAR content in the motor cortex of HET and KO mice at (C) P30 and (D) P50. (E) Densitometric analysis of Western blots of PAR. (F) NAD contents in the motor cortex of HET and KO mice at P30 and P50. Basal NAD content was 0.73 ± 0.12 μmol/g tissue. In (A), (C), and (D), each blot is representative of 6 animals per group. In (B), (E), and (F), each column represents the mean ± SEM of 6 animals per group