Fig. 5.

Effects of poly(adenosine diphosphate-ribose) polymerase (PARP) inhibitors on mitochondrial membrane potential in Ndufs4 knockout (KO) cultured glial cells. The effect of a 72-h treatment with N-(6-oxo-5,6-dihydrophenanthridin-2-yl)-(N,N-dimethylamino)acetamide hydrochloride (PJ34) (20 μM) or Olaparib (100 nM) on mitochondrial membrane potential [measured by means of potentiometric, fluorescent dye tetramethylrhodamine ethyl ester (TMRE)] of cultured glial cells from Ndufs4 KO mice is shown as (A) the mean ± SEM of 2 experiments conducted in triplicate and (B) a representative cytofluorimetric plot. *p < 0.05, **p < 0.01, vs control, analysis of variance plus Tukey’s post hoc test