Fig. 1.

A: effects of the Western (WES) diet on markers of cardiac hypertrophy compared with control (CON) hearts. Heart weight to tibia length (HW/TL), cardiomyocyte cross-sectional area (CSA), and representative images of cardiomyocyte size. Scale bar: 20 μm. Background and contrast were adjusted using ImageJ software (National Institutes of Health, Bethesda, MD). B: left ventricular homogenates from WES-fed mice exhibited higher protein levels of repressor element 1-silencing transcription factor (REST), α-sk Actin, and ANP vs. CON. Calsequestrin was the internal loading control. n = 6 per group except for CSA; n = 3 per group, *P < 0.05 vs. CON.