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Location of Plxna3 transcript in the neonate rat retina. Double in situ hybridisation (ISH) and immunofluorescence staining in P1 (A-C) and P7 retina (D-F). Expression was restricted mainly to the ganglion cell layer (GCL), with overlap of Plxna3 ISH and βIII-Tubulin positive putative retinal ganglion cells (C,F). GCL and dark arrows: ganglion cell layer; scale bars: 50 μm.
