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. 2014 Jul 1;3(7):e172. doi: 10.1038/mtna.2014.25

Figure 4.

Figure 4

In vivo luciferase expression profiles from pLive.Luc plasmids containing or not target sites for miR-122, with or without injection of AmiR122. Balb/c mice were hydrodynamically injected with 5 µg of plasmids and followed over time. Each value represents the average of the treated group (+SD). Plotted values are above the background signal of the IVIS machine of 1 × 105 photons/sec/cm2/sr. (a) Luciferase expression in mice injected with plasmids that lack (pLive.Luc) or contain target sites for miR-122 (pLive.Luc.122TS) (n ≥ 4). (b) Luciferase expression in mice co-injected with pLive.Luc and AmiR122 in comparison with expression from mice injected with pLive.Luc.122TS or pLive.Luc only. Expression was monitored for 8 days (n = 4). (c) Effect of AmiR122 administration on the expression from plasmids without miR-122 target sites. A number of eight animals were preinjected with pLive.Luc plasmid. After 6 months, four animals/group were hydrodynamically injected with either 20 nmol of AmiR122 in physiological salt solution (PSS) or with PSS only. (d) Effect of AmiR122 administration on the expression from plasmids with miR-122 target sites. A number of eight animals were preinjected with pLive.Luc.122TS plasmid. After 6 months, four animals/group were hydrodynamic injected with either 20 nmols of AmiR122 in PSS or with PSS only.