Isolation, culture and genetic manipulation of mouse pancreatic ductal cells

. Author manuscript; available in PMC: 2014 Aug 5.

Published in final edited form as: Nat Protoc. 2013 Jun 20;8(7):1354–1365. doi: 10.1038/nprot.2013.079


Amount/volume	Reagent	Final concentration

500 ml	DMEM/F12	—
25 ml	Nu-Serum IV	5%
5 ml	Penicillin-streptomycin	1%
4.2 ml	Bovine pituitary extract 3 mg ml⁻¹	25 μg ml⁻¹
2.5 ml	ITS⁺ premix	—
100 μl	Epidermal growth factor 100 μg ml⁻¹	20 ng ml⁻¹
50 μl	Cholera toxin 1 mg ml⁻¹	100 ng ml⁻¹
50 μl	3,3,5-Triiodo-L-thyronine, 50 μM	5 nM
5 μl	Dexamethasone	1 μM
2.5 g	Glucose	5 mg ml⁻¹
0.66 g	Nicotinamide	1.22 mg ml⁻¹