Reagent	μl per 4-well plate	Ratio (vol/vol)
L-Glutamine (29.2 mg ml 1, 200 mM, 100×)	294 μl	1:8.5
NaHCO3 7.5% solution	77 μ l	1:32.5
MEM, 10× (without glutamine)	245 μl	1:10.2
Nutragen (bovine collagen I, 6.0 mg ml−1)	816 μl	1:3.1
HEPES (1 M, pH 7.6)	49 μl	1:51
Sterile H2O	1,020 μl	1:2.5
Final volume	2,500 μl