Fig. 1.

Kidney blood flow occurs in a sequential spatiotemporal pattern. A–E: representative images of a tomato lectin (TL; green)-injected embryo (A) and kidneys (B–E) at various developmental stages. A: embryonic day 11.5 (E11.5) embryo showing perfused vessels throughout the head (white arrowheads) and body of the embryo, with the site of injection showing very bright staining (yellow arrowhead). B: dissected E11.5 kidney showing perfused vessels surrounding the developing kidney in a honeycomb arrangement (as marked by the dotted line). C: E13.5 kidney (as marked by the dotted line) showing perfusion of the major renal vessels (white arrowheads). Some lectin can also been seen sticking to the ureteric epithelium (yellow arrowhead). D: E15.5 kidney (as marked by the dotted line) showing perfusion of the smaller vascular branches (yellow arrowheads) as well as several perfused glomeruli (white arrowheads). E: E17.5 kidney showing significant perfusion throughout the developing kidney including numerous perfused glomeruli (white arrowheads) and smaller caliber vessels (yellow arrowheads).