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. 2014 Jun 11;307(3):F337–F345. doi: 10.1152/ajprenal.00208.2014

Fig. 7.

Fig. 7.

Varying O2 concentrations mediate the amount of nephron progenitor differentiation. A–F: Six2cre mice bred with a tdTomato reporter mouse (red) were grown under varying O2 concentrations for 7 days. A–C: E12.5 kidneys at 0 days of culture showing the kidneys placed in normoxia (A), intermediate hypoxia (B), and hypoxia (C) at developmentally comparable stages. There was a presence of nephron progenitors but no differentiated structures. D–F: after 7 days of culture in normoxia (C), the kidney showed numerous differentiated nephron structures (white arrowheads), whereas a stepwise reduction in the number of glomerular structures was observed in the intermediate (E) and hypoxic (F) conditions. G–I: representative whole mount images of PECAM staining in explants at various O2 concentrations. Under the normal O2 concentration (G), significant differentiation and patterning of vessels were observed. There was still relatively well-defined differentiation at 3% O2 concentration (H), but a complete lack of vascular organization was observed in the 1% O2 concentration (I).