Fig. 1.

Overexpression of APOL1 in human podocytes. Differentiated human podocytes were transduced with a lentivirus [titrated as 0.8 pg human immunodeficiency virus (HIV) p24 protein/cell] for 3 h and were cultured in fresh medium. Cell lysates were collected at 24 (A) and 48 (C) h postinfection (hpi) and were subjected to Western blot assay. B and D: quantification of the expression of APOL1 in A and C, respectively, and the results are means ± SD of 3 independent samples. V, vector alone; W, APOL1, wild-type (WT); G1, APOL1 G1 variant; G2, APOL1 G2 variant. *P < 0.05 compared with control (vector).