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. 2014 Jun 4;307(3):F326–F336. doi: 10.1152/ajprenal.00647.2013

Fig. 4.

Fig. 4.

APOL1 risk variants increase podocyte swelling. Human podocytes were transduced with the lentivirus (titrated as 0.8 pg HIV p24 protein/cell) for 3 h and were cultured in fresh medium. Cells were subjected to PI/Hoechst staining at 48 hpi. Representative microphotographs (A) were selected to show the apoptotic (green arrow), necrotic (white arrow), and secondary necrotic (yellow arrow) cells, and the ratios of these cells were calculated (B). Scale bars = 50 μm. *P < 0.05 compared with vector, while # P < 0.05 compared with APOL1WT.