Fig. 3.

Expression of wild-type and mutant 2.0/lacZ transgenes in the kidney. X-Gal staining of adult kidney and liver sections from two independent 2.0/lacZ founders (founders 11 and 14) and two independent 2.0m/lacZ founders (founders 54 and 63) is shown. A, B, D, and E: blue nuclei indicate transgene expression in renal collecting ducts in mice carrying the wild-type 2.0-kb promoter. C, F, I, and L: neither the wild-type nor mutant transgene was expressed in the liver. G, H, J, and K: no transgene expression was observed in kidneys from mice carrying the 2.0-kb promoter with a mutation of the HNF-1β-binding site located at position −49 bp. Scale bars = 50 μm.