Fig. 11.

ANG II-, AA-, and Gβ₁γ₂-induced fibronectin expression is dependent on c-Src. Cells were transfected (10 μg) with a plasmid carrying a c-Src dominant negative (DN-Src) construct or an empty vector before incubation with or without ANG II (1 μmol/l) or AA (15 μmol/l; A) or before transfection with plasmids encoding β₁ and γ₂, (5 μg each; B) for 48 h. Cell lysates were fractionated on SDS-PAGE, and fibronectin protein expression was assessed by direct immunoblotting of cell lysates (top). Actin was included as a control for loading and the specificity of change in protein expression. A representative blot from 4 independent experiments is shown. Bar graph at the bottom represents the ratio of the intensity of the fibronectin band quantified by densitometry factored by the densitometric measurements of the actin band. Values are means ± SE from 4 independent experiments. ***P < 0.001 vs. control. ^#P < 0.001 vs. DN-Src.