Fig. 2.

Endogenous expression of different Gβγ subunits. Rabbit proximal tubule cells were transiently transfected with empty vector (mock) or 10 μg each of the expression plasmids for Gβ₁, Gβ₂, Gβ₃, Gβ₄, or Gγ₂. Equal amounts of cell lysates (30 μg) were resolved by SDS-PAGE followed by Western blotting with the specified antibodies. Equal protein loading was confirmed by membrane reprobing with an anti-β-actin antibody. The blot shown is representative of 4 independent experiments.