Fig. 8.

Gβ₁γ₂-induced c-Src and ERK phosphorylation is eicosanoid independent. Serum-starved proximal tubular cells were transfected with Gβ₁γ₂ or empty vector (mock) for 24 h. Cell lysates were immunoblotted with anti-phospho-c-Src-Tyr⁴¹⁶ (A, top) or anti-phospho-ERK1/2 (p44/42 MAPK)-Thr²⁰²/Tyr²⁰⁴ (B, top) antibodies, and membranes were stripped and reprobed with anti-c-Src (A, bottom) or anti-ERK1/2 antibodies (B, bottom). Bar graphs depict the quantitative densitometry analysis for Western blot densitometry data. Values are means ± SE of 4 independent experiments. *P < 0.05 indicates a significant effect of Gβ₁γ₂ compared with empty vector (mock)-treated cells.