Fig. 9.

Class I HDAC activity is required for activation of renal Akt and STAT3 after FA-induced AKI. Kidney tissues were collected at 48 h after FA injection, with or without MS-275 administration. A: kidney tissue lysates were subjected to immunoblot analysis with specific antibodies against p-Akt, Akt, p-STAT3, STAT3, and GAPDH. B: expression levels of p-STAT3, STAT3, and α-tubulin were calculated by densitometry and the ratio between p-STAT3 and total STAT3 was determined. C: total STAT3 levels were normalized with GAPDH. D: ratio between p-Akt and total Akt was determined. E: total Akt levels were normalized with GAPDH. Data are means ± SE (n = 6). Representative immunoblots are 2 samples from 6 animals in each group. Means with different symbols are significantly different from one another (P < 0.05).