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. 2014 Jun 3;307(3):E245–E261. doi: 10.1152/ajpendo.00010.2014

Fig. 5.

Fig. 5.

Coexpression of p53 and ATF4 induces more muscle fiber atrophy than either transcription factor alone. A and B: TA muscles of C57BL/6 mice were transfected with 2 μg of p-eGFP plus either 20 μg of pcDNA3 (control), 10 μg of p-wt-p53 + 10 μg of pcDNA3 (p53), 10 μg of p-ATF4-FLAG + 10 μg of pcDNA3 (ATF4), or 10 μg of p-wt-p53 + 10 μg p-ATF4-FLAG (p53 + ATF4). Muscles were harvested 7 days posttransfection. A: representative images. B: mean fiber diameters; each data point represents the mean diameter of ≥200 fibers from 1 muscle. Horizontal bars denote means ± SE. Statistical analysis used 1-way ANOVA with Sidak's post hoc test; different letters are statistically different (P ≤ 0.05).