Fig. 4.

Metabolomic analyses of adipose tissue. Analyses of epididymal adipose tissue metabolites from WT mice fed a LFD or HFD for 6 wk. A: multivariate analysis; partial least squares-discriminant analysis (PLS-DA). Inset shows PLS-DA analysis from the uncorrected data set. B: hierarchial clustering; heat map and dendogram. C: univariate analysis; volcano plot of metabolites. Those metabolites that were significantly higher in abundance are in the quadrant shaded red, and those that were significantly lower are shaded green (P < 0.05, t-test). The identity of these metabolites is found in Table 1. The inset shows volcano plot analysis using data that were not corrected for protein content. D: metabolites found to be significantly different were subjected to pathway impact analysis using Metaboanalyst MetPA and the Mus musculus pathway library. Fisher's exact test was used for overrepresentation analysis, and relative betweenness centrality was used for pathway topology analysis; n = 14 animals (7 WT LFD and 7 WT HFD).