Fig. 1.
A: schematic of the myosin heavy chain (MHC)/rat leptin receptor (LepR) transgene. RT-PCR of RNA from the heart of control and transgenic MHC-LepR mice. B: representative real-time PCR for cardiac leptin receptor and 18s rRNA from control, MHC-LepR, db/db, and db/db-cardiac LepR rescue mice. Arrows indicate threshold cycles (CT) for 18s and LepR. Expression level is presented as the difference in CT between LepR and 18s compared with control mice. C: effect of acute leptin treatment on cardiac phosphorylated (p)-STAT3 and STAT3 levels. Mice were injected with 100 μg of leptin, and hearts were harvested 1 h later. *P < 0.05 compared with db/db; n = 3.